Product Description

mSin3A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90886-50, BS90886-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96ST3(Human) Q60520(Mouse)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50
FC:1:50-1:100

Background: It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (alternatively designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad-binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: mSin3A polyclonal antibody detects endogenous levels of mSin3A protein.

Bioworld Molecular Weight: 145 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of mSin3A on 293T cell using anti-mSin3A antibody at 1/1,000 dilution., ICC staining mSin3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: SIN3A, AW553200, DKFZP434K2235, FLJ90319, Histone deacetylase complex subunit Sin 3a, Histone deacetylase complex subunit Sin3a, KIAA0700, Kiaa4126, mKIAA4126, Paired amphipathic helix protein Sin 3a, Paired amphipathic helix protein Sin3a, Sin 3a, SIN3 homolog A, SIN3 homolog A transcription regulator (yeast), SIN3 homolog A transcription regulator, SIN3 transcription regulator homolog A, Sin3a, SIN3A protein, SIN3A, Transcriptional co repressor Sin 3A, Transcriptional co repressor Sin3A, Transcriptional corepressor Sin3a, Transcriptional regulator SIN3A,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified