Product Description

MT-ND3 Polyclonal Antibody

Catalogue Numbers: BS60188-50, BS60188-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P03897

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by trans-location of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 3 (ND3) localizes to the hydrophobic protein fragment of complex I. Mutations in the gene encodiing for ND3 may be associated with Parkinson disease.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MT-ND3 polyclonal antibody detects endogenous levels of MT-ND3 protein.

Extra Notes: Western blot (WB) analysis of MT-ND3 pAb at 1:500 dilution
Lane1:HepG2 whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(40ug)
Lane4:SGC7901 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 13 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: NADH-ubiquinone oxidoreductase chain 3
; EC=1.6.5.3; NADH dehydrogenase subunit 3; MT-ND3; MTND3; NADH3; ND3

Immunogen: A synthetic peptide corresponding to residues in Human MT-ND3.

Conjugate: Unconjugated

Modification: Unmodified