MTCO2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90888-50, BS90888-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P00403(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Cytochrome c oxidase subunit II (COX2), also designated COII, MTCO2 or oxidative phosphorylation (OxPhos) complex IV, subunit II, is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electrochemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded. Defects in COX2 are associated with tumor formation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MTCO2 polyclonal antibody detects endogenous levels of MTCO2 protein.
Bioworld Molecular Weight: 25 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MTCO2 on different lysates using anti-MTCO2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: THP-1
Lane 3: Hela, ICC staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MT-CO2, COII, COX2, COX2, COXII, Cytochrome c oxidase II, Cytochrome c oxidase polypeptide II, Cytochrome c oxidase subunit 2, MT CO2, MT-CO2, MTCO2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified