Product Description

MUC13 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67022-50, BS67022-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q9H3R2

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF/ICC

All Applications: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Background: Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of MUC13 protein.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MUC13 expression in KNRK (A) whole cell lysates., Immunohistochemical analysis of MUC13 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of MUC13 staining in KNRK cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: DRCC1; RECC; Mucin-13; MUC-13; Down-regulated in colon cancer 1

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MUC13. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified