MUC6 Monoclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: MB66085-50, MB66085-100 Product: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Swiss-Prot: Q6W4X9 Host: Mouse Reactivity: Human Applications: IHC All Applications: IHC (1/100 - 1/300) Background: Mucin 6 (also designated MUC 6 and gastric mucin) is a large glycoprotein that plays a major role in the protection of the gastrointestinal tract. Mucin 6 carries GlcNAca1Æ4GalβÆR structures, indicating that a1,4-N-acetylglucosaminyltransferase is important to the formation of the mucous glycoproteins in vivo. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileum and right colon. In gastric cancer, Mucin 6 has an altered expression. In normal stomach, Mucin 6 apomucin is associated with Lewis type 2; Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deeper mucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation. Mucin 6 mRNA is expressed transiently in the nephrogenic zone of the kidney in the early mid-trimester of development, and Mucin 6 glycoprotein is expressed in the epithelium of ureteric buds and collecting ducts, but absent from adult kidney. Proliferating bile ductular cells express Mucin 6 apomucin in diseased liver, especially in chronic viral hepatitis with active necroinflammation, suggesting that this secreted mucin acts as a cytoprotective agent and represents a phenotype of reactive biliary epithelium in chronic viral hepatitis. Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Extra Notes: Immunohistochemical analysis of MUC6 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Bioworld Molecular Weight: ~ 257 kDa Specificity: Recognizes endogenous levels of MUC6 protein. Alternative Name: Mucin-6; MUC-6; Gastric mucin-6 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human MUC6. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified