Product Description

MUC6 Polyclonal Antibody

Catalogue Numbers: BS60178-50, BS60178-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q6W4X9

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Mucin 6 (also designated MUC 6 and gastricmucin) is a large glycoprotein that plays amajor role in the protection of the gastrointestinal tract. MUC6 carries GlcNAcα1→4Galβ→R structures, indicating thatα1, 4-N-acetylglucosaminyltransferase isimportant to the formation of the mucous glycoproteinsin vivo. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileumand rightcolon. In gastriccancer, Mucin 6 has an altered expression. In normalstomach, Mucin 6 apomucin is associated with Lewistype 2; Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deepermucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation. Mucin 6mRNA is expressed transientlyin the nephrogeniczone of the kidneyin the early mid-trimester of development, and Mucin 6 glycoprotein is expressed in the epithelium of ureteric buds and collecting ducts, but absent fromadultkidney. Proliferating bile ductularcells express Mucin 6 apomucin in diseased liver, especially in chronicviral hepatitiswith active necroinflammation,suggesting that this secreted mucin acts as a cytoprotective agent and represents a phenotype of reactive biliary epithelium in chronicviral hepatitis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MUC6 polyclonal antibody detects endogenous levels of MUC6 protein.

Extra Notes: Western blot (WB) analysis of MUC6 pAb at 1:500 dilution
Lane1:HCT116 whole cell lysate(40ug)
Lane2:Aml-12 whole cell lysate(40ug)
Lane3:Hela whole cell lysate(40ug)
Lane4:A2780 whole cell lysate(40ug)
Lane5:The Fallopian tube tissue lysate of Mouse(40ug) Lane6:HepG2 whole cell lysate (40ug)

Bioworld Molecular Weight: ~ 270 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Mucin-6; MUC-6; Gastric mucin-6; MUC6

Immunogen: A synthetic peptide corresponding to residues in Human MUC6.

Conjugate: Unconjugated

Modification: Unmodified