Multiplex Assay Kit for Kelch Repeat And BTB Domain Containing Protein 10 (KBTBD10), etc. by FLIA (Flow Luminescence Immunoassay)
Size: 96T
Catalogue Number: LML640Ra-96
Citations, Manuals and MSDS Available upon request.
Detection range: 0.0098-10ng/mL
Application: FLIA Kit for Antigen Detection.
Organism species: Rattus norvegicus (Rat)
Sensitivity: The minimum detectable dose of this kit is typically less than 0.0033ng/mL
Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3h
Method: Double-antibody Sandwich
Specificity: This assay has high sensitivity and excellent specificity for detection of Kelch Repeat And BTB Domain Containing Protein 10 (KBTBD10), etc. by FLIA (Flow Luminescence Immunoassay).No significant cross-reactivity or interference between Kelch Repeat And BTB Domain Containing Protein 10 (KBTBD10), etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kelch Repeat And BTB Domain Containing Protein 10 (KBTBD10), etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kelch Repeat And BTB Domain Containing Protein 10 (KBTBD10), etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Assay procedure summary: 1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle: Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed isproportional to the concentration of analytes of interest in the sample.
Alternative Names: KRP1; KBTBD10; Sarcosin; Sarcomeric Muscle Protein; Kelch-like protein 41; Kel-like protein 23; Kelch-related protein 1
Item Name: Kelch Repeat And BTB Domain Containing Protein 10
Research Field: Signal transduction;
Research Use Only