Multiplex Assay Kit for Non Metastatic Cells 2, Protein NM23B Expressed In (NME2), etc. by FLIA (Flow Luminescence Immunoassay)
Size: 96T
Catalogue Number: LMJ816Hu-96
Citations, Manuals and MSDS Available upon request.
Detection range: 0.01-10ng/mL
Application: FLIA Kit for Antigen Detection.
Organism species: Homo sapiens (Human)
Sensitivity: The minimum detectable dose of this kit is typically less than 0.003 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 3h
Method: Double-antibody Sandwich
Specificity: This assay has high sensitivity and excellent specificity for detection of Non Metastatic Cells 2, Protein NM23B Expressed In (NME2), etc. by FLIA (Flow Luminescence Immunoassay).No significant cross-reactivity or interference between Non Metastatic Cells 2, Protein NM23B Expressed In (NME2), etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Non Metastatic Cells 2, Protein NM23B Expressed In (NME2), etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Non Metastatic Cells 2, Protein NM23B Expressed In (NME2), etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Assay procedure summary: 1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
Test principle: Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer.The MFI developed isproportional to the concentration of analytes of interest in the sample.
Alternative Names: NDPKB; NM23-H2; NM23B; Puf; Nucleoside diphosphate kinase B; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB
Item Name: Non Metastatic Cells 2, Protein NM23B Expressed In
Research Field: Signal transduction;
Research Use Only