Myc-Tag Monoclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-20007-60, E-AB-20007-120, E-AB-20007-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: Myc-Tag
Target Synonym: c Myc; c Myc Epitope Tag; c Myc Tag; Myc Epitope Tag; Myc proto-oncogene protein; Transcription factor p64
Research Areas: Tags and Cell Markers
Conjugation: Unconjugated
Host: Mouse
Application: WB, IF, IP
Isotype: IgG
Clonality: Monoclonal
Clone No.: Clone:3B8
Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Concentration: 1 mg/mL
Immunogen: Synthetic Peptide
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method: Protein A purification
Dilution: WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only