Product Description

Myc-tag Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB8024-50, MB8024-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01106

Host: Mouse

Reactivity: Transfected

Applications: WB

All Applications: WB: 1:500~1:1000

Background: A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Myc-tag monoclonal antibody detects over-expressed or recombinant proteins containing the Myc tag.

Immunogen: Synthetic peptide, corresponding to Human Myc-tag.

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Myc-tag monoclonal antibody at 1:500 dilution
Lane1:HEK293T whole cell lysate(40ug)
Lane2:Myc-GRB2 tagged protein(5uL)