Product Description

Myc-Tag Mouse Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: AP1026M-50, AP1026M-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with mμltiple subunits.The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Category: Primary Antibody

Reactivity: Transfected

Host: Mouse

Applications: WB, IHC, IF, IP

Alternate Names: Myc

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Synthetic peptide Myc.

Dilution: WB: 1:20000~1:100000
IHC: 1:50~1:200
IF: 1:200~1:500
IP: 1:50~1:200

Purification: The antibody was affinity-purified from cell cμlture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE).

Specificity: Myc-Tag Monoclonal Antibody detects over-expressed or recombinant proteins containing the myc epitope tag.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product: Mouse IgG1, 0.5mg/ml in PBS with 0.05% proclin300, 50% glycerol, pH7.2

Research Use Only