Product Description

Myc-tag Polyclonal Antibody

Catalogue Numbers: BS67271-50, BS67271-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Host: Rabbit

Applications: WB, IP

All Applications: WB (1/1000 - 1/2000), IP (1/10 - 1/100)

Background: A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes the Myc-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Extra Notes: Western blot analysis of Anti-Myc-tag Antibody against HEK293T cells transfected with vector overexpressing Myc tag (A) and untransfected (B).

Bioworld Molecular Weight: N/A

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: c Myc; c Myc Epitope Tag; c Myc Tag; Myc Epitope Tag; Myc proto-oncogene protein; Transcription factor p64

Immunogen: KLH-conjugated synthetic peptide EQKLISEEDL.

Conjugate: Unconjugated

Modification: Unmodified