Myeloperoxidase Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90902-50, BS90902-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P05164(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: The heme protein myeloperoxidase (MPO) is a major component of azurophilic granules of neutrophils and polymorphonuclear leukocytes. Optimal oxygen-dependent microbiocidal activity depends on MPO as the critical enzyme for the generation of hypochlorous acid and other toxic oxygen products. The MPO precursor is synthesized during the promyelocytic stage of myeloid differentiation and is subsequently processed and transported intracellularly to the lysosomes. The precursor undergoes cotranslational N-linked glycosylation to produce a glycoprotein. Glucosidases in the endoplasmic reticulum (ER) or early cis Golgi convert the pro-MPO to a form which is sorted into a prelysosomal compartment, which undergoes final proteolytic maturation to native MPO, a pair of heavy-light protomers. In normal neutrophils, MPO is expressed as a dimer. Calreticulin, a calcium-binding protein residing in the ER, interacts specifically with fully glycosylated apopro-MPO. iMPO mRNA is abundant in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60 cells. MPO is expressed at high levels in circulating neutrophils and monocytes but is not detectable in microglia, brain-specific macrophages or normal brain tissue.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Myeloperoxidase polyclonal antibody detects endogenous levels of Myeloperoxidase protein.
Bioworld Molecular Weight: 84 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Myeloperoxidase on HL-60 cell lysates using anti-Myeloperoxidase antibody at 1/1,000 dilution., ICC staining Myeloperoxidase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MPO, 84 kDa myeloperoxidase, 89 kDa myeloperoxidase, EC 1.11.1.7, EC1.11.2.2, fj80f04, MPO, mpx, myeloid-specific peroxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain, PERM, wu:fj80f04,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified