Myf-6 (R150) Polyclonal Antibody-BS1956

Myf-6 (R150) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1956-50, BS1956-100

Sizes: 50µl, 100µl

Swiss-Prot: P23409

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Myf-6 (R150) polyclonal antibody detects endogenous levels of Myf-6 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Myf-6 (R150) pAb at 1:1000 dilution
Lane 1: THP-1 whole cell lysate (40ug)
Lane 2: MG63 whole cell lysate (20ug)
Lane 3: PC3 whole cell lysate (10ug)
Lane 4: H9C2 whole cell lysate (40ug)
Lane 5: SP2/0 whole cell lysate (20ug), Immunohistochemistry (IHC) analyzes of Myf-6 (R150) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 30 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Myogenic factor 6; Myf-6; Class C basic helix-loop-helix protein 4; bHLHc4; Muscle-specific regulatory factor 4; MYF6; BHLHC4; MRF4

Immunogen: Synthetic peptide, corresponding to amino acids 120-170 of Human Myf-6.

Conjugate: Unconjugated

Modification: Unmodified