MYH6/α-MHC Polyclonal Antibody-BS72402

MYH6/α-MHC Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS72402-50, BS72402-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P13533

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:1000 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 250kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Mouse skeletal muscle, using MYH6/α-MHC Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunofluorescence analysis of mouse heart cells using MYH6/α-MHC Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining., Immunofluorescence analysis of rat heart cells using MYH6/α-MHC Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: MYH6; ASD3; CMD1EE; CMH14; MYHC; MYHCA; SSS3; alpha-MHC; myosin-6

Immunogen: Recombinant fusion protein of human MYH6/α-MHC(NP_002462.2).

Conjugate: Unconjugated

Modification: Unmodified