Product Description

MYH9 Polyclonal Antibody

Catalogue Numbers: BS60434-50, BS60434-100

Sizes: 50µl, 100µl

Alternative Name: Myosin-9; Cellular myosin heavy chain, type A; Myosin heavy chain 9; Myosin heavy chain, non-muscle Iia; Non-muscle myosin heavy chain A; NMMHC-A; Non-muscle myosin heavy chain Iia; NMMHC II-a; NMMHC-IIA; MYH9

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35579

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of 2 heavy chains (MHC) and 4 light chains (MLC) that interacts with actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Myosin heavy chain 9 can also be designated Myosin IIa, nonmuscle myosin heavy chain IIa, Cellular myosin heavy chain, type A, myosin-9 or NMMHC-IIA. Myosin heavy chain 9 is involved in cell shape, cytokinesis, and specialized functions such as capping and secretion. It is expressed in leukoctyes and in kidney glomeruli. Defects in the MYH9 gene, which encodes myosin heavy chain 9, are linked to Sebastian syndrome (SBS), Fechtner syndrome (FTNS), Alport syndrome with macrothrombocytopenia (APSM), autosomal dominant nonsyndromic sensorineural deafness 17 (DFNA17) and Epstein syndrome (EPS).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MYH9 polyclonal antibody detects endogenous levels of MYH9 protein.

Bioworld Molecular Weight: ~ 230 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MYH9 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate
Lane 2: Rat testis tissue lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human MYH9.

Conjugate: Unconjugated

Modification: Unmodified