MYL2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90904-50, BS90904-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P10916(Human) P51667(Mouse) P08733(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat, zebrafish
Applications: WB, IHC, IP,
All Applications: WB:1:1,000-1:5,000
IHC:1:50-1:200
Background: Encoded by the MYL2 gene, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, also designated MLC-2 or MLC2v, is part of a hexameric complex of two heavy chains and four light chains predominantly expressed in adult cardiac ventricle muscle. Myosin regulatory light chain 2 binds calcium and has been shown to be a useful molecular marker for cardiac chamber specification. The co-expression of myosin regulatory light chain 7 (MYL7) and myosin regulatory light chain 2 in the outflow tract and atrioventricular canal, together with the single expression in the atrial (MYL7) and ventricular (MYL2) myocardium, permits the delineation of their boundaries. At the amino acid level there is 96% homology between the human and mouse myosin regulatory light chain sequences. Mutations in MYL2 are correlated with mid-left ventricular chamber type hypertrophic cardiomyopathy (MVC2) as well as familial hypertrophic cardiomyopathy type 10 (CMH10).
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MYL2 polyclonal antibody detects endogenous levels of MYL2 protein.
Bioworld Molecular Weight: 19 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Myosin Light Chain 2 on different lysates using anti-Myosin Light Chain 2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse liver
Lane 2: Mouse brain, Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
Alternative Name: MLC-2, MLC-2v, MLC-2s/v, MYL2, Cardiac myosin light chain-2, Cardiac ventricular myosin light chain 2, CMH10, MLC 2v, MLC-2, MLC-2v, MLC2, MLRV, MYL 2, MYL2, Myosin light chain 2 regulatory cardiac slow, Myosin light polypeptide 2 regulatory cardiac slow, Myosin regulatory light chain 2, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform, Regulatory light chain of myosin, RLC of myosin, Slow cardiac myosin regulatory light chain 2, ventricular/cardiac muscle isoform,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified