Product Description

MYL3 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS8335-50, BS8335-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Myosin, the major component of thick muscle filaments, is a long asymmetric molecμle containing a globμlar head and a long tail. Activation of smooth and cardiac/ventricμlar muscle primarily involves pathways which increase calcium and myosin phosphorylation, resμlting in contraction. Myosin in vertebrate striated muscle is composed of two heavy chains and four light chains. There are two distinct types of light chains: the phosphorylatable, regμlatory or MLC2 type, and the nonphosphorylatable, alkali or MLC1 and MLC3 types. Myosin light chain phosphatase acts to regμlate muscle contraction by de-phosphorylating activated myosin light chain. The role of myosin alkali light chains in vertebrate skeletal muscle is poorly understood, although alkali light chains in smooth muscle may be involved with the active site of myosin. Several isoforms of myosin alkali light chains have been identified, encoded by a family of myosin light chain genes. Each is associated with different muscle types. Human myosin light chain can be used as a cardiac marker. Myosin light chain 3, encoded by MYL3, is an alkali light chain also referred to as both the ventricμlar isoform (MLC1v) and slow skeletal muscle isoform. Myosin light chain 3 proteins in human and mouse share 91% sequence identity overall.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF

Alternate Names: Myosin light chain 3, Cardiac myosin light chain 1, CMLC1, Myosin light chain 1, slow-twitch muscle B/ventricμlar isoform, MLC1SB, Ventricμlar/slow twitch myosin alkali light chain, MYL3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fμll length Human MYL3.

Dilution: WB: 1:500~1:2000
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MYL3 polyclonal antibody detects endogenous levels of MYL3 protein.

Molecular Weight: ~ 22 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P08590

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only