Product Description

MYL7 Polyclonal Antibody

Catalogue Numbers: BS5808-50, BS5808-100

Sizes: 50µl, 100µl

Alternative Name: Myosin regulatory light chain 2, atrial isoform; MLC-2a; MLC2a; Myosin light chain 2a; Myosin regulatory light chain 7; MYL2A; MYLC2A

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q01449

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Encoded by the MYL7 gene, myosin regulatory light chain 7, also designated Myosin regulatory light chain 2, atrial isoform (MLC-2a), is part of a hexameric complex of two heavy chains and four light chains predominantly expressed in adult atrial muscle. Myosin regulatory light chain 7 binds calcium and has been shown to be a useful molecular marker for cardiac chamber specification. The co-expression of myosin regulatory light chain 7 and myosin light chain 2 (MLC2v) in the outflow tract and atrioventricular canal, together with the single expression in the atrial (MYL7) and ventricular (MYL2) myo-cardium, permits the delineation of their boundaries. At the amino acid level there is 95% homology between the human and mouse myosin regulatory light chain 7 sequences.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MYL7 polyclonal antibody detects endogenous levels of MYL7 protein.

Bioworld Molecular Weight: ~ 19 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MYL7 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 130-175 of Human MYL7.

Conjugate: Unconjugated

Modification: Unmodified