MYLK Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90905-50, BS90905-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q15746(Human) Q6PDN3(Mouse) Unigene:203004(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:2,000-1:10,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:50-1:100
Background: MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MYLK polyclonal antibody detects endogenous levels of MYLK protein.
Bioworld Molecular Weight: 110 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MYLK on human lung lysates using anti-MYLK antibody at 1/5,000 dilution., ICC staining MYLK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MLCK, smMLCK, KRP, MYLK, deglutamylated form, DKFZp686I10125, EC 2.7.11.18, FLJ12216, Kinase related protein, Kinase-related protein, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK, MYLK, MYLK1, Myosin light chain kinase, Myosin light polypeptide kinase, OTTHUMP00000180642, OTTHUMP00000180643, smMLCK, smooth muscle, Smooth muscle myosin light chain kinase, Telokin,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified