Myoglobin Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS75296-50, BS75296-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P02144
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB
All Applications: WB,1:500 - 1:2000
Background: This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Extra Notes: Western blot analysis of extracts of various cell lines, using Myoglobin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Note: For research use only, not for use in diagnostic procedure.
Bioworld Molecular Weight: 15kDa
Alternative Name: MB; PVALB; myoglobgin; myoglobin
Immunogen: RecoMyoglobin inant fusion protein containing a sequence corresponding to amino acids 1-154 of human Myoglobin(NP_005359.1).
Conjugate: Unconjugated
Modification: Unmodified