Product Description

Myoglobin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7593-50, BS7593-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P02144

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 17KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using Myoglobin antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunofluorescence analysis of rat skeletal muscle cells using Myoglobin Rabbit pAb at dilution of 1:300. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse skeletal muscle cells using Myoglobin Rabbit pAb at dilution of 1:300. Blue: DAPI for nuclear staining.

Alternative Name: MB; PVALB; myoglobgin; myoglobin

Immunogen: RecoMyoglobin inant fusion protein containing a sequence corresponding to amino acids 1-1-154 of human Myoglobin (NP_005359.1).

Conjugate: Unconjugated

Modification: Unmodified