Product Description

Myosin 14 Polyclonal Antibody

Catalogue Numbers: BS62405-50, BS62405-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: Q7Z406

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively. While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions. The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization. Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration.

Purification and Purity: The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Myosin 14 polyclonal antibody detects endogenous levels of Myosin 14 protein.

Extra Notes: Western blot (WB) analysis of Myosin 14 polyclonal antibody at 1:500 dilution
LaneA:H1792 whole cell lysate
LaneB:HEK293T whole cell lysate
LaneC:HepG2 whole cell lysate
LaneD:The liver tissue lysate of Mouse
LaneE:The liver tissue lysate of Rat

Bioworld Molecular Weight: ~ 228 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Myosin-14; Myosin heavy chain 14; Myosin heavy chain, non-muscle Iic; Non-muscle myosin heavy chain Iic; NMHC II-C; MYH14; KIAA2034; FP17425

Immunogen: Synthetic peptide, corresponding to Human Myosin 14.

Conjugate: Unconjugated

Modification: Unmodified