Product Description

Myosin-8 Polyclonal Antibody

Catalogue Numbers: BS60181-50, BS60181-100

Sizes: 50µl, 100µl

Alternative Name: Myosin-8; Myosin8; Myosin 8; Myosin heavy chain 8; Myosin heavy chain, skeletal muscle, perinatal; MyHC-perinatal; MYH8

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P13535

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Myosin-8 polyclonal antibody detects endogenous levels of Myosin-8 protein.

Bioworld Molecular Weight: ~ 223 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Myosin-8 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: sp2/0 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human Myosin-8.

Conjugate: Unconjugated

Modification: Unmodified