Product Description

MYPT3 Polyclonal Antibody

Catalogue Numbers: BS62438-50, BS62438-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot: Q96I34

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: The members of the MYPT family, MYPT1, MYPT2 and MYPT3 are the myosinbinding subunits of myosin phosphatase and an integral component of the myosin protein phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. MYPT1 is localized on stress fibers and is distributed close to the cell membrane and at cell-cell contacts to regulate myosin phosphatase activity. In addition to MYPT1, a novel isoform of MYPT1, MYPT2, also interacts with PPlc. MYPT3, also designated PP16A, inhibits protein phosphatase activity involving phosphorylase, myosin light chain and myosin substrates. It acts as a lipid anchor and binds PP1. MYPT3 localizes primarily to the cell membrane.

Purification and Purity: The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.

Specificity: MYPT3 polyclonal antibody detects endogenous levels of MYPT3 protein.

Extra Notes: Western blot (WB) analysis of MYPT3 polyclonal antibody at 1:500 dilution
LaneA:HEK293T whole cell lysate
LaneB:A2780 whole cell lysate
LaneC:HCT116 whole cell lysate
LaneD:H9C2 whole cell lysate
LaneE:CT-26 whole cell lysate

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Protein phosphatase 1 regulatory subunit 16A; Myosin phosphatase-targeting subunit 3; PPP1R16A; MYPT3

Immunogen: Synthetic peptide, corresponding to Human MYPT3.

Conjugate: Unconjugated

Modification: Unmodified