Product Description

MYT1 Polyclonal Antibody

Catalogue Numbers: BS62249-50, BS62249-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99640

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MYT1 polyclonal antibody detects endogenous levels of MYT1 protein.

Extra Notes: Western blot (WB) analysis of MYT1 polyclonal antibody at 1:500 dilution
LaneA:The Liver tissue lysate of Rat
LaneB:The Muscle tissue lysate of Rat

Bioworld Molecular Weight: ~ 70, 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase; PKMYT1; MYT1

Immunogen: Synthetic peptide, corresponding to Human MYT1.

Conjugate: Unconjugated

Modification: Unmodified