Product Description

MZF-1 (D10) Polyclonal Antibody

Catalogue Numbers: BS1972-50, BS1972-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P28698

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Zinc finger genes encode metal-binding proteins are transcriptional regulators of other genes. Myeloid zinc finger 1 (MZF-1), also designated zinc finger protein 42, and transcription factor ZBP-89, also designated zinc finger protein 148, belong to the Krüppel C2H2-type zinc-finger protein family. The gene encoding for the MZF-1 protein maps to chromosome 19q13.43, while the gene encoding for ZBP-89 is localized on chromosome 3q21.2. These proteins are nuclear proteins involved in the regulation of transcriptional events. MZF-1 regulates transcription during hemopoietic development and plays a role in myeloid cell differentiation. It regulates the CD34 promoter in a tissue-specific manner. MZF-1 and FHL3 can form a complex of high molecular mass with other proteins in the nucleus. MZF-1 is induced by retinoic acid and is primarily expressed in differentiating myeloid cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MZF-1 (D10) pAb detects endogenous levels of MZF-1 protein.

Extra Notes: Western blot (WB) analysis of MZF-1 (D10) pAb at 1:500 dilution
Lane1:MG63 whole cell lysate(30ug)
Lane2:Panc1 whole cell lysate(40ug)
Lane3:C6 whole cell lysate(40ug)
Lane4:3T3-L1 whole cell lysate(40ug)
Lane5:K562 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 82 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Myeloid zinc finger 1; MZF-1; Zinc finger and SCAN domain-containing protein 6; Zinc finger protein 42; MZF1; MZF; ZNF42; ZSCAN6

Immunogen: Synthetic peptide, corresponding to amino acids 5-55 of Human MZF-1.

Conjugate: Unconjugated

Modification: Unmodified