Product Description

N4BP1 (Y415) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3340-50, BS3340-100

Sizes: 50µl, 100µl

Swiss-Prot: O75113

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin–protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73α, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73α for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73α and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: N4BP1 (Y415) polyclonal antibody detects endogenous levels of N4BP1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of N4BP1 (Y415) pAb at 1:1000 dilution
Lane 1: A2780 whole cell lysate (20ug)
Lane 2: A549 whole cell lysate (20ug)
Lane 3: HEK293 whole cell lysate (20ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of N4BP1 (Y415) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 100 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: NEDD4 binding protein 1; N4BP1; KIAA0615

Immunogen: Synthetic peptide, corresponding to amino acids 391-440 of Human N4BP1.

Conjugate: Unconjugated

Modification: Unmodified