Product Description

N4BP2L2 Polyclonal Antibody

Catalogue Numbers: BS5850-50, BS5850-100

Sizes: 50µl, 100µl

Alternative Name: NEDD4-binding protein 2-like 2; Phosphonoformate immuno-associated protein 5; CG005; PFAAP5

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92802

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: N4BP2L2 (NEDD4-binding protein 2-like 2), also known as PFAAP5 (phosphonoformate immuno-associated protein 5), is a 583 amino acid nuclear protein that potentially is involved in transcriptional regulation. N4BP2L2 is phosphorylated on Ser 199 in response to DNA damage, probably by ATM or ATR. Primarily expressed in bone marrow, N4BP2L2 is dramatically down-regulated after exposure to arsenic compounds, an event which precedes neutropenia. PFAAP5 interacts with both Gfi-1 and Neutrophil Elastase, two proteins that are implicated in neutropenia disorders. Defects in the gene encoding Neutrophil Elastase, ELA2, are the cause of cyclic haematopoiesis, which, with decreased numbers of circulating neutrophils, leads to an increased risk for opportunistic infection.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: N4BP2L2 polyclonal antibody detects endogenous levels of N4BP2L2 protein.

Bioworld Molecular Weight: ~ 67 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of N4BP2L2 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: H9C2 cell lysate
Lane 3: Rat lung tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 400-445 of Human N4BP2L2.

Conjugate: Unconjugated

Modification: Unmodified