Product Description

Na+/K+-ATPase α1 (phospho-S16) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4259-50, BS4259-100

Swiss-Prot: P05023

Host: Rabbit

Na+/K+-ATPase α1 (phospho-S16) Polyclonal Antibody-BS4259

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium / potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.The different isoforms of the sodium / potassium ATPase exhibit tissue specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Na+/K+-ATPase α1 (S16) polyclonal antibody detects endogenous levels of Na+/K+-ATPase α1 protein only when phosphorylated at Ser16.

Bioworld Molecular Weight: ~ 113 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-Na+/K+-ATPase α1 (S16) pAb at 1:2000 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: HEK293T treated with EGF (100ng/ml,15 minutes) whole cell lysate
Lane 3: HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate
Lane 4: C6 whole cell lysate
Lane 5: NIH-3T3 whole cell lysate, Immunohistochemistry (IHC) analyzes of p-Na+/K+-ATPase α1 (S16) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Alternative Name: Sodium/potassium-transporting ATPase subunit alpha-1; Short name=Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1; ATP1A1

Immunogen: Synthetic phosphopeptide derived from human Na+/K+-ATPase α1 around the phosphorylation site of Serine 16.

Modification: Phosphorylation

Conjugate: Unconjugated