Na+/K+-ATPase α1 (Y10) Polyclonal Antibody-BS1436

Na+/K+-ATPase α1 (Y10) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1436-50, BS1436-100

Sizes: 50µl, 100µl

Swiss-Prot: P05023

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IF

Background: The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium / potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.The different isoforms of the sodium / potassium ATPase exhibit tissue specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Na+/K+-ATPase α1 (Y10) polyclonal antibody detects endogenous levels of A Na+/K+-ATPase α1 protein

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Na+/K+-ATPase α1 (Y10) pAb at 1:500 dilution
Lane 1: HCT116 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: SGC7901 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Na+/K+-ATPase α1 (Y10) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 112 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Sodium/potassium-transporting ATPase subunit alpha-1; Short name=Na (+)/k (+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1; ATP1A1

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Na+/K+-ATPase α1.

Conjugate: Unconjugated

Modification: Unmodified