Product Description

NACA1 (Phospho-S43) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64574-50, BS64574-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13765

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NACA1 (Phospho-S43) polyclonal antibody detects endogenous levels of NACA1 protein only when phosphorylated at Ser43.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of NACA1 (Phospho-S43) polyclonal antibody at 1:500 dilution
Lane A: HEK293T whole cell lysate
Lane B: Jurkat whole cell lysate
Lane C: NIH3T3 whole cell lysate
Lane D: CT26 whole cell lysate
Lane E: H9C2 whole cell lysate
Lane F: PC12 whole cell lysate

Alternative Name: HSD48; NAC-alpha; NACA1; skNAC

Immunogen: Synthetic phosphopeptide derived from human NACA1 around the phosphorylation site of Serine 43.

Conjugate: Unconjugated

Modification: Phosphorylation