Nanog Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90910-50, BS90910-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9H9S0(Human)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Nanog (from “Tir Na Nog,” the mythologic Celtic land of the ever young) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression have been detected by Northern analysis in the undifferentiated NTERA-2 cl.D1 embryonal carcinoma cell line.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Nanog polyclonal antibody detects endogenous levels of Nanog protein.
Bioworld Molecular Weight: 37 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Nanog on F9 cell lysates using anti-Nanog antibody at 1/1,000 dilution., ICC staining Nanog in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: hNanog, NANOG, Embryonic stem cell specific homeobox protein (Nanog), ENK, FLJ12581, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, NANOG, Nanog homeobox, NANOG,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified