Native Pseudomonas sp. Protocatechuate 3, 4-dioxygenase:
Powder. 3.0 U/mg
Size: 1 KU
Catalogue Number: DIA-214
Citations, Manuals and MSDS Available upon request.
Product Description: In enzymology, a protocatechuate 3,4-dioxygenase (EC 1.13.11.3) is an enzyme that catalyzes the chemical reaction: 3,4-dihydroxybenzoate + O2 ā 3-carboxy-cis,cis-muconate. Thus, the two substrates of this enzyme are 3,4-dihydroxybenzoate (protocatechuic acid) and O2, whereas its product is 3-carboxy-cis,cis-muconate. This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O2 as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, iron.
Storage: -20Ā°C
Applications: This enzyme is useful for enzymatic determination of choline esterase when coupled with p-hydroxybenzoate hydroxylase.
Activity: Gradeā ¢ 3.0U/mg-solid or more (containing approx. 40% of stabilizers)
CAS#: 9029-47-4
Research Use Only