Product Description

NBL1 Polyclonal Antibody

Catalogue Numbers: BS5694-50, BS5694-100

Sizes: 50µl, 100µl

Alternative Name: Neuroblastoma suppressor of tumorigenicity 1; DAN domain family member 1; Protein N03; Zinc finger protein DAN; DAN; DAND1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P41271

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: The DAN (differential screening-selected gene aberrative in neuroblastoma) protein family contains antagonists of bone morphogenetic protein (BMP) signaling that are expressed in the neural crest. All family members are secreted proteins that act as BMP antagonists in embryonic explants and are expressed in the proximal airway epithelium of the lung during embryonic development. This family includes the head-inducing factor Cerberus, the dorsaling factor gremlin and the tumor suppressor DAN. DAN, also known as zinc finger protein DAN, NO3, DAND1 or neuroblastoma suppressor of tumorigenicity 1 (NBL1), is a secreted protein containing one C-terminal cysteine knot-like (CTCK) domain. It is produced in small neurons of the dorsal root ganglion and its expression is activated by MATH-1. In addition to antagonizing BMP signaling, DAN also antagonizes the action of TGFβ. DAN is a possible tumor suppressor for human neuroblastoma; defects may result in or contribute to its progression. DAN may also be a neuromodulator in inflammatory pain.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NBL1 polyclonal antibody detects endogenous levels of NBL1 protein.

Bioworld Molecular Weight: ~ 19 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NBL1 polyclonal antibody at 1:500 dilution
Lane 1: Jurkat cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 135-180 of Human NBL1.

Conjugate: Unconjugated

Modification: Unmodified