Product Description

NCAPH Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS78574-50, BS78574-100

Swiss-Prot: Q15003

Host: Rabbit

NCAPH Polyclonal Antibody-BS78574

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:5000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 85kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using NCAPH antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s., Immunofluorescence analysis of U2OS cells using NCAPH Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: NCAPH; BRRN1; CAP-H

Immunogen: Recombinant fusion protein of human NCAPH(NP_056156.2).

Modification: Unmodified

Conjugate: Unconjugated