Product Description

NCE2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11269-50, MB11269-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q969M7

Host: Rabbit

Reactivity: Human

Applications: WB,IHC-F,IHC-P,ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NCE2 in Hela, MCF-7Lysates using NCE2 antibody.

Bioworld Molecular Weight: Calculated MW: 21 kDa; Observed MW: 21 kDa

Specificity: IgG

Alternative Name: NCE2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2

Immunogen: Recombinant protein of human NCE2

Conjugate: Unconjugated

Modification: Unmodified