NCF4 Monoclonal Antibody-MB12176

NCF4 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12176-50, MB12176-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q15080

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 39 kDa; Observed MW: 39 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of p40phox in HL-60, U251 lysates using NCF4 antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil using p40phox antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunocytochemistry analysis of p4phox in hela using p4phox antibody, and DAPI

Alternative Name: NCF; CGD3; P40PHOX; SH3PXD4

Immunogen: A synthetic peptide of human p40-phox

Conjugate: Unconjugated

Modification: Unmodified