NCK1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90917-50, BS90917-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P16333(Human) Q99M51(Mouse) B2RZ33(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IF, IHC, IP, FC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50
Background: The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKα) and NCK2 (NCKβ), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr 1009); overexpression of Nck2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2. The human NCK1 and NCK2 genes map to chromosomes 3q22.3 and 2q12.2, respectively.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: NCK1 polyclonal antibody detects endogenous levels of NCK1 protein.
Bioworld Molecular Weight: 43/35 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 1: NIH-3T3
Lane 2: Rat kidney tissue
Lane 3: Mouse testis tissue, ICC staining NCK1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: Nck-1, NCK1, NCK, Cytoplasmic protein NCK1, Melanoma Nck protein, MGC12668, NCK 1, NCK adaptor protein 1, NCK alpha, NCK, NCK tyrosine kinase, Nck-1, NCK1, NCK1, NCKalpha, Non catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK alpha, SH2/SH3 adaptor protein NCK-alpha,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified