Product Description

NCOA3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77222-50, BS77222-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y6Q9

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using NCOA3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5min.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 155kDa

Alternative Name: NCOA3; ACTR; AIB-1; AIB1; CAGH16; CTG26; KAT13B; RAC3; SRC-3; SRC3; TNRC14; TNRC16; TRAM-1; bHLHe42; pCIP

Immunogen: A synthetic peptide of human NCOA3(NP_858045.1).

Conjugate: Unconjugated

Modification: Unmodified