Product Description

NCOA5 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67243-50, BS67243-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9HCD5

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IHC

Background: Nuclear receptor coregulator that can have both coactivator and corepressor functions. Interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF-2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. Involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC in response to 17-beta-estradiol (E2).

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 204% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of NCOA5 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NCOA5 expression in mouse muscle (A), rat muscle (B) whole cell lysates., Immunohistochemical analysis of NCOA5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 63 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: KIAA1637; Nuclear receptor coactivator 5; NCoA-5; Coactivator independent of AF-2; CIA

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NCOA5. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified