Product Description

ND4L Polyclonal Antibody

Catalogue Numbers: BS1633-50, BS1633-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P03903

Host: Rabbit

Reactivity: Mouse

Applications: WB

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 4L (ND4L) is most likely a component of the hydrophobic protein fragment of Complex I. Mutations in the gene encodiing for ND4 are implicated in Leber hereditary optic neuropathy, a rare condition that can cause loss of central vision.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ND4L polyclonal antibody detects endogenous levels of ND4L protein.

Extra Notes: Western blot (WB) analysis of ND4L polyclonal antibody at 1:1000 dilution
Lane1:NIH/3T3 whole cell lysate(48μg)

Bioworld Molecular Weight: ~ 10 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: NADH-ubiquinone oxidoreductase chain 4L (EC:1.6.5.3); NADH dehydrogenase subunit 4L; Mtnd4l; mt-Nd4l; Nd4l

Immunogen: A synthetic peptide corresponding to amino acids 80-97 of Mouse ND4L.

Conjugate: Unconjugated

Modification: Unmodified