Product Description

NDP52 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS70796-50, BS70796-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13137

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 52kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of mouse pancreas, using NDP52 antibody at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s., Immunofluorescence analysis of PC-12 cells using NDP52 Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.

Alternative Name: CALCOCO2; NDP52

Immunogen: Recombinant fusion protein of human NDP52(NP_005822.1).

Conjugate: Unconjugated

Modification: Unmodified