NDUFA8 (P141) Polyclonal Antibody-BS3336

NDUFA8 (P141) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3336-50, BS3336-100

Sizes: 50µl, 100µl

Swiss-Prot: P51970

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: NDUFA8 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: NDUFA8 (P141) polyclonal antibody detects endogenous levels of NDUFA8 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of NDUFA8 (P141) polyclonal antibody at 1:500 dilution
Lane 1: C6 whole cell lysate (40ug)
Lane 2: AML-12 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: L02 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of NDUFA8 (P141) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing strong cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 22 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; Complex I-19kD; CI-19kD; Complex I-PGIV; CI-PGIV; NADH-ubiquinone oxidoreductase 19 kDa subunit; NDUFA8

Immunogen: Synthetic peptide, corresponding to amino acids 120-170 of Human NDUFA8.

Conjugate: Unconjugated

Modification: Unmodified