NDUFS3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90921-50, BS90921-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O75489(Human)
Host: Rabbit
Reactivity: Human
Applications: WB, IHC ICC IF, IP,
All Applications: WB:1:500
IHC:1:50
ICC:1:50
IP:1:10-1:50
Background: NDUFS3 (NADH dehydrogenase ubiquinone iron-sulfur protein 3) is one of about 45 subunits comprising complex I of the oxidative phosphorylation electron transport chain. The multisubunit NADH: ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of the mitochondria. NDUFS3 is the last subunit of the seven subunits that make up the core of complex I. Through use of chaotropic agents, complex I can be separated into three different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction includes NDUFS1-7. NDUFS3 contains a highly conserved casein kinase II phosphorylation site. Mutations in the NDUFS3 gene may cause optic atrophy, Leigh syndrome and complex I deficiency.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: NDUFS3 polyclonal antibody detects endogenous levels of NDUFS3 protein.
Bioworld Molecular Weight: 25 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of NDUFS3 on HepG2 cell using anti-NDUFS3 antibody at 1/500 dilution., ICC staining NDUFS3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: CI-30kD, NDUFS3, CI 30, CI 30KD, CI-30kD, Complex I 30KD, Complex I 30kDa subunit, COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT, Complex I-30kD, mitochondrial, NADH coenzyme Q reductase, NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa, NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, NADH dehydrogenase ubiquinone 30 kDa subunit, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH-Ubiquinone Oxidoreductase Fe-S Protein 3, NDUFS3, NDUS3,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified