Product Description

NDUFV1 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS71051-50, BS71051-100

Swiss-Prot: P49821

Host: Rabbit

NDUFV1 Polyclonal Antibody-BS71051

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF, IHC ELISA

All Applications: WB: 1:500~1:2000
IHC: 1:100~1:500
IF: 1:100~1:500
ELISA: 1:5000~1:10000

Background: The mitochondrial respiratory chain provides energy to cells via oxidative phosphorylation and consists of four membrane-bound electron-transporting protein complexes (I-IV) and an ATP synthase (complex V). This gene encodes a 51 kDa subunit of the NADH:ubiquinone oxidoreductase complex I; a large complex with at least 45 nuclear and mitochondrial encoded subunits that liberates electrons from NADH and channels them to ubiquinone. This subunit carries the NADH-binding site as well as flavin mononucleotide (FMN)- and Fe-S-biding sites. Defects in complex I are a common cause of mitochondrial dysfunction; a syndrome that occurs in approximately 1 in 10,000 live births. Mitochondrial complex I deficiency is linked to myopathies, encephalomyopathies, and neurodegenerative disorders such as Parkinson's disease and Leigh syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: NDUFV1 polyclonal antibody detects endogenous levels of NDUFV1 protein.

Bioworld Molecular Weight: ~ 49 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Primary: Anti- NDUFV1 at 1/1000 dilution, Paraformaldehyde-fixed, paraffin embedded (Mouse brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NDUFV1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

Alternative Name: UQOR1; CI-51K; CI51KD

Immunogen: KLH conjugated synthetic peptide derived from human NDUFV1: 41-140/464

Modification: Unmodified

Conjugate: Unconjugated