Product Description

NEDD4-2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90924-50, BS90924-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96PU5(Human) Q8CFI0(Mouse) Unigene:72824(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IP

All Applications: WB:1:1,000-1:5,000

Background: NEDD4-L (neural precursor cell expressed, developmentally down-regulated 4-like), also known as RSP5, NEDD4-2 or NEDL3, is a 975 amino acid protein that localizes to the cytoplasm and contains four WW domains, one HECT domain and one C2 domain. Expressed ubiquitously with highest expression in pancreas, prostate and kidney, NEDD4-L functions as an E3 ubiquitin-protein ligase that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme and transfers that ubiquitin residue to substrates targeted for degradation. Through its ability to ubiquitinate and induce the proteasome-dependent degradation of proteins such as Smad2 and TGFβ RII, NEDD4-L is thought to inhibit the TGFβ signaling pathway, thereby regulating the signaling pathways that control cell growth and differentiation. NEDD4-L is expressed as eight isoforms due to alternative splicing events.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NEDD4-2 on Jurkat cellsLysates using anti-NEDD4-2 antibody at 1/1,000 dilution.

Bioworld Molecular Weight: 112 kDa

Specificity: NEDD4-2 polyclonal antibody detects endogenous levels of NEDD4-2 protein.

Alternative Name: NEDD4L, E3 ubiquitin protein ligase NEDD4 like protein, E3 ubiquitin-protein ligase NEDD4-like, KIAA0439, NED4L, NEDD4 2, NEDD4 2c, Nedd4-2, NEDD4-2a, NEDD4.2, NEDD4b, NEDD4L, NEDD4La, NEDD4Lb, NEDD4Lc, NEDD4Ld, NEDD4Le, NEDD4Lf, NEDD4Lg, NEDD4Lh, NEDL3, Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase, Neural precursor cell expressed, developmentally down regulated 4 like, RSP5, Ubiquitin protein ligase Rsp5,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified