Product Description

Neddylin (D21) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1463-50, BS1463-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: NEDD8 is a ubiquitin (Ub)-like molecμle that shares 80% homology with ubiquitin, a protein known to modify and target various proteins for proteolytic degradation. NEDD8 and the corresponding yeast homolog RUB1 are activated by the E1 ubiquitin activating enzyme UBA2, which forms isopeptide linkages between thio esters. Similar to the Ub-mediated proteolytic pathway, NEDD8 is covalently coupled to an E3 Ub ligase by the E2 conjugating enzyme, UBC12. NEDD8 preferentially associates with the Cμl-2 protein in the E3 ligase complex CBCVHL, which consists of cμllin proteins associating with elongin B/C and the tumor suppressor F-box protein von Hippel Lindau (VHL). NEDD8 is predominantly localized to the nucleus and is highly expressed in adμlt heart and skeletal muscle. In vitro studies indicate that NEDD8 tetramers are also able to bind to the 26S proteasome, and that they assemble into complexes with conserved Ub-like moieties, suggesting that NEDD8 may regμlate proteolysis of intracellμlar proteins similar to other Ub-mediated pathways.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regμlated protein 8, NEDD-8, Ubiquitin-like protein Nedd8, NEDD8

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human Neddylin.

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Neddylin (D21) polyclonal antibody detects endogenous levels of Neddylin protein.

Molecular Weight: ~ 9 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q15843

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only