Product Description

NEFL Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66089-50, MB66089-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubμles. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits. Similar in structure to other intermediate filament proteins, neurofilaments have a globμlar amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament. While neurofilaments are critical for radial axon growth and determine axon caliber, microtubμles are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly. Research studies have shown neurofilament accumμlations in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS).

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: IHC

Alternate Names: NF68, NFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human NEFL. The exact sequence is proprietary.

Dilution: IHC (1/100 - 1/300)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of NEFL protein.

Molecular Weight: ~ 61 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P07196

Product: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only