Product Description

NEIL1 Polyclonal Antibody

Catalogue Numbers: BS62370-50, BS62370-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96FI4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: NEIL1, NEIL2 and NEIL3, also known as Endonuclease VIII-like 1, 2 and 3 or DNA-(apurinic or apyrimidinic site) lyase NEIL 1, 2 and 3, are nuclear proteins involved in the repair of DNA damaged by oxidation. The NEIL proteins belong to the FPG familiy of proteins. They act as DNA glycosylases that can recognize and remove damaged bases, leaving an abasic site. NEIL3, however, lacks the proline residue at the N-terminus which acts as the active site residue found in NEIL1 and NEIL2. NEIL1 is a ubiquitiously expressed protein that is upregulated during S phase. NEIL2 is expressed primarily in testis, heart, skeletal muscle, placenta, brain, kidney and liver while NEIL3 is detected primarily in thymus and testis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: NEIL1 polyclonal antibody detects endogenous levels of NEIL1 protein.

Extra Notes: Western blot (WB) analysis of NEIL1 polyclonal antibody at 1:500 dilution
LaneA:PC12 whole cell lysate
LaneB:AML-12 whole cell lysate
LaneC:MCF-7 whole cell lysate
LaneD:A549 whole cell lysate

Bioworld Molecular Weight: ~ 47 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Endonuclease 8-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei homolog 1; NEH1; Nei-like protein 1; NEIL1

Immunogen: Synthetic peptide, corresponding to Human NEIL1.

Conjugate: Unconjugated

Modification: Unmodified